tonbo cytek pe cy7 cd25 pc61 5 Search Results


pe cd25  (Cytek Biosciences)
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Cytek Biosciences pe cd25
Pe Cd25, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd25 pc61 5  (Cytek Biosciences)
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a,b) . Flow cytometry quantification validating that M-II KO mice have decreased MHC-II expression in tumour-infiltrating immune cells (CD45 + ), including macrophages (Mϕ, CD45 + CD11b + Ly6G F4/80 + ), dendritic cells (DC, CD45 + CD11b + Ly6G − F4/80 − CD11c + ), and B cells (CD45 + B220 + ) (CTRL: n=10; M-II KO : n=11). c) . Flow cytometry gating of suspension cells dissociated from thymus, characterized as CD45 + EpCAM − immune cells and CD45 − EpCAM + epithelial cells. d,e) . Quantification of MHC-II expression of thymus showing that MHC-II expression is inhibited in immune cells but preserved in epithelial cells in M-II KO mice (CTRL: n=7; M-II KO : n=6). f) . Quantification of different types of tumour-infiltrating stroma cells (n=10/group). g) . Quantification of MHC-II expression in different cell types (CTRL: n=10; Tie2Cre;H2Ab +/floxP : n=5; M-II KO : n=11). h) . Quantification of T cells in spleens from 5 – 6 week-old female mice showing the number of T cells is independent of MHC-II expression on Tie2Cre + cells (CTRL: n=7; M-II KO : n=6). i) . Quantification of activated CD4 + -TLs and effector CD4 + -TLs from tumours of similar sizes. (activated CD4 + -TL: CD45 + CD3 + CD4 + <t>CD25</t> + FoxP3 − Treg: CD45 + CD3 + CD4 + CD25 + FoxP3 + ; Effector memory cell: CD44 + CD62L − Naïve CD4 + -TL: CD44 − CD62L + ) (n=11/group). j) . The percentages of CD4 + -TL activation markers in spleen showing a similar pattern as in tumour (i) (CTRL: n=7; M-II KO : n=6). k) . Quantification of different E0771 tumour-infiltrating T helper cells (IFNγ + Th1, IL4 + Th2 and IL17A + Th17) (n=11/group). l) . Quantification of E0771 tumour-infiltrating CD4 + -TL cells, macrophage, dendritic cells, B cells and neutrophils (CD45 + CD11b + Ly6G high ) (n=11/group). Data are presented as means ± s.e.m. The genetic backgrounds of mice are denoted with different colors shown on the right of (l) . WT, Tie2Cre and H2Ab floxP/floxP were combined as CTRL group. P values were calculated using two-tailed unpaired Student’s t-test (a–b, d–f, h–k) or two-tailed unpaired Mann–Whitney U test ( l ). n.s., not significant.
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violetfluor 450 coupled anti cd25 pc61 5  (Cytek Biosciences)
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a,b) . Flow cytometry quantification validating that M-II KO mice have decreased MHC-II expression in tumour-infiltrating immune cells (CD45 + ), including macrophages (Mϕ, CD45 + CD11b + Ly6G F4/80 + ), dendritic cells (DC, CD45 + CD11b + Ly6G − F4/80 − CD11c + ), and B cells (CD45 + B220 + ) (CTRL: n=10; M-II KO : n=11). c) . Flow cytometry gating of suspension cells dissociated from thymus, characterized as CD45 + EpCAM − immune cells and CD45 − EpCAM + epithelial cells. d,e) . Quantification of MHC-II expression of thymus showing that MHC-II expression is inhibited in immune cells but preserved in epithelial cells in M-II KO mice (CTRL: n=7; M-II KO : n=6). f) . Quantification of different types of tumour-infiltrating stroma cells (n=10/group). g) . Quantification of MHC-II expression in different cell types (CTRL: n=10; Tie2Cre;H2Ab +/floxP : n=5; M-II KO : n=11). h) . Quantification of T cells in spleens from 5 – 6 week-old female mice showing the number of T cells is independent of MHC-II expression on Tie2Cre + cells (CTRL: n=7; M-II KO : n=6). i) . Quantification of activated CD4 + -TLs and effector CD4 + -TLs from tumours of similar sizes. (activated CD4 + -TL: CD45 + CD3 + CD4 + <t>CD25</t> + FoxP3 − Treg: CD45 + CD3 + CD4 + CD25 + FoxP3 + ; Effector memory cell: CD44 + CD62L − Naïve CD4 + -TL: CD44 − CD62L + ) (n=11/group). j) . The percentages of CD4 + -TL activation markers in spleen showing a similar pattern as in tumour (i) (CTRL: n=7; M-II KO : n=6). k) . Quantification of different E0771 tumour-infiltrating T helper cells (IFNγ + Th1, IL4 + Th2 and IL17A + Th17) (n=11/group). l) . Quantification of E0771 tumour-infiltrating CD4 + -TL cells, macrophage, dendritic cells, B cells and neutrophils (CD45 + CD11b + Ly6G high ) (n=11/group). Data are presented as means ± s.e.m. The genetic backgrounds of mice are denoted with different colors shown on the right of (l) . WT, Tie2Cre and H2Ab floxP/floxP were combined as CTRL group. P values were calculated using two-tailed unpaired Student’s t-test (a–b, d–f, h–k) or two-tailed unpaired Mann–Whitney U test ( l ). n.s., not significant.
Violetfluor 450 Coupled Anti Cd25 Pc61 5, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25 pc61 5 apc  (Cytek Biosciences)
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(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Cd25 Pc61 5 Apc, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd25 pc61 5 percp c5 5 tonbo biosciences  (Cytek Biosciences)
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(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Anti Cd25 Pc61 5 Percp C5 5 Tonbo Biosciences, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25  (Cytek Biosciences)
94
Cytek Biosciences cd25
(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Cd25, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc anti cd25 pc61 5  (Cytek Biosciences)
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(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Fitc Anti Cd25 Pc61 5, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tonbo cytek pe cy7 cd25 pc61 5  (Cytek Biosciences)
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(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Tonbo Cytek Pe Cy7 Cd25 Pc61 5, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse cd25  (Cytek Biosciences)
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Cytek Biosciences anti mouse cd25
(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Anti Mouse Cd25, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25  (Bio X Cell)
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Bio X Cell cd25
(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Cd25, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7  (Cytek Biosciences)
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Cytek Biosciences pe cy7
(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as <t>CD25</t> + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).
Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a,b) . Flow cytometry quantification validating that M-II KO mice have decreased MHC-II expression in tumour-infiltrating immune cells (CD45 + ), including macrophages (Mϕ, CD45 + CD11b + Ly6G F4/80 + ), dendritic cells (DC, CD45 + CD11b + Ly6G − F4/80 − CD11c + ), and B cells (CD45 + B220 + ) (CTRL: n=10; M-II KO : n=11). c) . Flow cytometry gating of suspension cells dissociated from thymus, characterized as CD45 + EpCAM − immune cells and CD45 − EpCAM + epithelial cells. d,e) . Quantification of MHC-II expression of thymus showing that MHC-II expression is inhibited in immune cells but preserved in epithelial cells in M-II KO mice (CTRL: n=7; M-II KO : n=6). f) . Quantification of different types of tumour-infiltrating stroma cells (n=10/group). g) . Quantification of MHC-II expression in different cell types (CTRL: n=10; Tie2Cre;H2Ab +/floxP : n=5; M-II KO : n=11). h) . Quantification of T cells in spleens from 5 – 6 week-old female mice showing the number of T cells is independent of MHC-II expression on Tie2Cre + cells (CTRL: n=7; M-II KO : n=6). i) . Quantification of activated CD4 + -TLs and effector CD4 + -TLs from tumours of similar sizes. (activated CD4 + -TL: CD45 + CD3 + CD4 + CD25 + FoxP3 − Treg: CD45 + CD3 + CD4 + CD25 + FoxP3 + ; Effector memory cell: CD44 + CD62L − Naïve CD4 + -TL: CD44 − CD62L + ) (n=11/group). j) . The percentages of CD4 + -TL activation markers in spleen showing a similar pattern as in tumour (i) (CTRL: n=7; M-II KO : n=6). k) . Quantification of different E0771 tumour-infiltrating T helper cells (IFNγ + Th1, IL4 + Th2 and IL17A + Th17) (n=11/group). l) . Quantification of E0771 tumour-infiltrating CD4 + -TL cells, macrophage, dendritic cells, B cells and neutrophils (CD45 + CD11b + Ly6G high ) (n=11/group). Data are presented as means ± s.e.m. The genetic backgrounds of mice are denoted with different colors shown on the right of (l) . WT, Tie2Cre and H2Ab floxP/floxP were combined as CTRL group. P values were calculated using two-tailed unpaired Student’s t-test (a–b, d–f, h–k) or two-tailed unpaired Mann–Whitney U test ( l ). n.s., not significant.

Journal: Nature

Article Title: Mutual Regulation of Tumour Vessel Normalization and Immunostimulatory Reprogramming

doi: 10.1038/nature21724

Figure Lengend Snippet: a,b) . Flow cytometry quantification validating that M-II KO mice have decreased MHC-II expression in tumour-infiltrating immune cells (CD45 + ), including macrophages (Mϕ, CD45 + CD11b + Ly6G F4/80 + ), dendritic cells (DC, CD45 + CD11b + Ly6G − F4/80 − CD11c + ), and B cells (CD45 + B220 + ) (CTRL: n=10; M-II KO : n=11). c) . Flow cytometry gating of suspension cells dissociated from thymus, characterized as CD45 + EpCAM − immune cells and CD45 − EpCAM + epithelial cells. d,e) . Quantification of MHC-II expression of thymus showing that MHC-II expression is inhibited in immune cells but preserved in epithelial cells in M-II KO mice (CTRL: n=7; M-II KO : n=6). f) . Quantification of different types of tumour-infiltrating stroma cells (n=10/group). g) . Quantification of MHC-II expression in different cell types (CTRL: n=10; Tie2Cre;H2Ab +/floxP : n=5; M-II KO : n=11). h) . Quantification of T cells in spleens from 5 – 6 week-old female mice showing the number of T cells is independent of MHC-II expression on Tie2Cre + cells (CTRL: n=7; M-II KO : n=6). i) . Quantification of activated CD4 + -TLs and effector CD4 + -TLs from tumours of similar sizes. (activated CD4 + -TL: CD45 + CD3 + CD4 + CD25 + FoxP3 − Treg: CD45 + CD3 + CD4 + CD25 + FoxP3 + ; Effector memory cell: CD44 + CD62L − Naïve CD4 + -TL: CD44 − CD62L + ) (n=11/group). j) . The percentages of CD4 + -TL activation markers in spleen showing a similar pattern as in tumour (i) (CTRL: n=7; M-II KO : n=6). k) . Quantification of different E0771 tumour-infiltrating T helper cells (IFNγ + Th1, IL4 + Th2 and IL17A + Th17) (n=11/group). l) . Quantification of E0771 tumour-infiltrating CD4 + -TL cells, macrophage, dendritic cells, B cells and neutrophils (CD45 + CD11b + Ly6G high ) (n=11/group). Data are presented as means ± s.e.m. The genetic backgrounds of mice are denoted with different colors shown on the right of (l) . WT, Tie2Cre and H2Ab floxP/floxP were combined as CTRL group. P values were calculated using two-tailed unpaired Student’s t-test (a–b, d–f, h–k) or two-tailed unpaired Mann–Whitney U test ( l ). n.s., not significant.

Article Snippet: The following antibodies against mouse antigens were used: anti-NG2 (1E6.4) and anti-EpCAM (caa7-9G8) (both from Miltenyi); anti-CD31 (MEC13.3), anti-CD45.1 (A20), anti-CD45.2 (104), anti-IFNγ (XMG1.2), anti-IL4 (11B11), anti-IL17A (TC11-18H10.1), anti-FoxP3 (MF14), anti-MHC-II (M5/114.15.2) (all from Biolegend); anti-CD25 (eBio7D4), anti-CD3ε (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53-6.7) (all from eBioscience); anti-B220 (RA3-6B2), anti-CD11b (M1/70), anti-CD11c (N418), anti-CD25 (PC61.5), anti-CD44 (IM7), anti-CD45 (30-F11), anti-CD62L (MEL14), anti-F4/80 (BM8) (all from Tonbo); anti-SiglecF (E50-2440, BD Biosciences).

Techniques: Flow Cytometry, Expressing, Suspension, Activation Assay, Two Tailed Test, MANN-WHITNEY

(A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as CD25 + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).

Journal: bioRxiv

Article Title: Role of Lamin A/C on dendritic cell function in antiviral immunity

doi: 10.1101/2024.05.14.593747

Figure Lengend Snippet: (A) Subcutaneous inoculation (s.c.) with 10 5 p.f.u. of VACV was administered to both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells, as well as CD25 + Foxp3 + Treg cells, was conducted in popliteal lymph nodes (pLN) at 6 and 14 days post-infection. The percentage of Th1 cells, CD8 CTL T cells, and Treg cells was assessed. The data represent means ± SEM from at least 8 mice per group, obtained from 3 independently conducted experiments, and were analyzed using unpaired Student’s t-test between genotypes at each time point. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant). (B) Intraperitoneal inoculation (i.p.) with 10 6 p.f.u. of VACV was performed on both WT and LysM-Lmna -/- mice. Flow cytometry analysis of IFNγ-producing CD4 and CD8 T cells was conducted in mesenteric lymph nodes (mLN) 6 days post-infection. The percentage of Th1 and CD8 CTL cells was determined. Data represent means ± SEM of at least 6 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is indicated by asterisks (*P < 0.05; **P < 0.01). (C) Infection with 10 5 p.f.u. of VACV through skin scarification (s.s.) in the tail was performed on WT and LysM-Lmna -/- mice. Flow cytometry analysis of the percentages of IFNγ-producing CD4 and CD8 T cells was conducted in extracted inguinal lymph nodes (iLN) at 6 and 14 days post-infection. (D) Viral load measurement in the tail skin was performed at 6 and 14 days post-infection. The data represent means ± SEM of at least 4 mice per group, derived from 2 independently conducted experiments, and were analyzed by unpaired Student’s t-test. Statistical significance is denoted by asterisks (*P < 0.05; **P < 0.01; ns, not significant).

Article Snippet: Antibodies against: CD4 (Clone RM4-5, GK1.5) -v450, -APC and PE; CD25 (PC61.5) -APC; CD69 (H1.2F3)-FITC; CD28 (37.51); CD3 (145-2C11); CD45.1 (A20)-Pe-Cy7, -v450, -FITC; CD45.2 (104) -v450, -redFluor710; CD11b (M1/70)-v450; CD86 GL-1 PE; IFNγ XMG1.2 FITC, -APC; Foxp3 (3G3)-FITC; MHC-II (I-A/I-E, M5/115.15.2)-APC; GR1 (RB6-8C5)-Biotin; CD80 (16-10A1)-Biotin; B220 (RA3-6B2)-Biotin; F4/80 (BM8.1)-Biotin; CD11b (M1/70)-Biotin; CD19 (1D3)-Biotin; CD25 (PC61.5)-Biotin from Tonbo.

Techniques: Flow Cytometry, Infection, Derivative Assay

Flow cytometry was employed to assess the percentage of activated CD4 T cells, specifically gated on CD25 + or CD69 + cells; Th1-differentiated cells, specifically gated on IFNγ+ cells; and proliferating CellTrace Violet + T cells. Maturation of GM-CSF-derived WT- and LysM-Lmna -/- -BMDCs was undertaken with (A) sonicated VACV extract, (B) Pam3CSK4, or (C) lipopolysaccharide (LPS), and, in all conditions, pulsed with OVA323–339 cognate OT-IIp. (D) WT- or Lmna -/- -BMDCs, matured with LPS and pulsed with the OVA 323–339 cognate OT-II peptide, were s.c. inoculated into CD45.1 OT-II recipient mice. Six days later, pLNs were extracted, and IFNγ-producing CD4 T cells were analyzed by flow cytometry. The data represent means ± SEM and are presented from a representative experiment out of 3 (A and B, n=3) or out of 4 (C, n=4) or out of 2 (D, n=4). Activation data were analyzed using One Way ANOVA and Bonferroni post-test, and proliferation and Th1 differentiation were assessed by unpaired Student’s t-test. Statistical significance was denoted by asterisks (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant).

Journal: bioRxiv

Article Title: Role of Lamin A/C on dendritic cell function in antiviral immunity

doi: 10.1101/2024.05.14.593747

Figure Lengend Snippet: Flow cytometry was employed to assess the percentage of activated CD4 T cells, specifically gated on CD25 + or CD69 + cells; Th1-differentiated cells, specifically gated on IFNγ+ cells; and proliferating CellTrace Violet + T cells. Maturation of GM-CSF-derived WT- and LysM-Lmna -/- -BMDCs was undertaken with (A) sonicated VACV extract, (B) Pam3CSK4, or (C) lipopolysaccharide (LPS), and, in all conditions, pulsed with OVA323–339 cognate OT-IIp. (D) WT- or Lmna -/- -BMDCs, matured with LPS and pulsed with the OVA 323–339 cognate OT-II peptide, were s.c. inoculated into CD45.1 OT-II recipient mice. Six days later, pLNs were extracted, and IFNγ-producing CD4 T cells were analyzed by flow cytometry. The data represent means ± SEM and are presented from a representative experiment out of 3 (A and B, n=3) or out of 4 (C, n=4) or out of 2 (D, n=4). Activation data were analyzed using One Way ANOVA and Bonferroni post-test, and proliferation and Th1 differentiation were assessed by unpaired Student’s t-test. Statistical significance was denoted by asterisks (*P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant).

Article Snippet: Antibodies against: CD4 (Clone RM4-5, GK1.5) -v450, -APC and PE; CD25 (PC61.5) -APC; CD69 (H1.2F3)-FITC; CD28 (37.51); CD3 (145-2C11); CD45.1 (A20)-Pe-Cy7, -v450, -FITC; CD45.2 (104) -v450, -redFluor710; CD11b (M1/70)-v450; CD86 GL-1 PE; IFNγ XMG1.2 FITC, -APC; Foxp3 (3G3)-FITC; MHC-II (I-A/I-E, M5/115.15.2)-APC; GR1 (RB6-8C5)-Biotin; CD80 (16-10A1)-Biotin; B220 (RA3-6B2)-Biotin; F4/80 (BM8.1)-Biotin; CD11b (M1/70)-Biotin; CD19 (1D3)-Biotin; CD25 (PC61.5)-Biotin from Tonbo.

Techniques: Flow Cytometry, Derivative Assay, Sonication, Activation Assay